Lab Techniques

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Nikon Fluorescence Microscope for Comet assay analysis

Comet Assay Analysis

Oxidative DNA damage is evaluated using the Comet assay. To quantitate the Comet assay, prepared samples are examined under a Nikon fluorescence microscope. Fifty to one hundred comets per slide are visually scored according to the amount of DNA present in the tail. The Olive tail moment = [(fluorescence intensity of the tail)/(fluorescence intensity of the head) x tail length], is used as a measure of DNA damage. (Klaunig JE)

TECAN Microplate Reader

TECAN Microplate Reader

TEAC

The Tecan Infinite M200 Microplate Reader is used to determine the amount of anti-oxidant in the sample. Magellan6 software converts instrument-generated WSP files to Excel spreadsheet format. Values are compared to standard curves. (Klaunig JE)

Fluorescent Microscope

Fluorescence Microscope 

Fluorescence Microscopy – Nuclear Activity Assays

Fluorescence microscopy is used to analyze nuclear activity assays.

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Cell culture hood

Cell Culture

“Cell culture involves the dispersal of cells in an artificial environment composed of nutrient solutions, a suitable surface to support the growth of cells, and ideal conditions of temperature, humidity, and gaseous atmosphere. In such a system, a researcher can precisely measure the response of the cell’s alterations in culture alterations, prospective drugs, the presence or absence of other kinds of cells, carcinogenic agents, and viruses.” (Coriell Institue for Cellular Research)

Dr. Zemin Wang

Dr. Zemin Wang using RT-PCR technology

Real-time PCR

“qPCR and, more specifically, real-time qPCR has become a routine and robust approach for measuring the expression of genes of interest, validating microarray experiments, and monitoring biomarkers.” (Biotechniques)

 

HPLC

Animal models

Pathology

Ultracentrifuge